Recombinant inbred lines
Recombinant inbred lines (RILs) are mapping population, which can be maintained eternally as seeds.
The F2 genotypes generated between homo lines are fixed by self-crossing. The conserved recombination patterns become a powerful tool in genome analysis, such as gene mapping. Also, phenotypic variations inherent in RILs allow an accurate genetic analysis as a consequence of feasibility of strain dependent traits evaluation and repetitive experiments.
"LjMG RILines" are RILs between Miyakojima MG-20 and Gifu B-129. These RILs were established at Kazusa DNA Res. Inst., by self-crossing 8 times the F2 seeds provided by Kawaguchi (The Univ. Tokyo).
In 2004, they have completed establishing 205 lines.
Currently, the two parents of "LjMG RILines", Miyakojima MG-20 and Gifu B-129, are the most commonly used experimental accessions. Kawaguchi has established the accession Miyakojima MG-20 by self-crossing 7 times the collection from Miyakojima among the Southwest Islands. This accession is characterized by the short generation time. On the other hand, Gifu B-129 was the first one to be established as an experimental accession, collected by Hirayoshi (Kyoto Univ.), named Gifu B-129 by Grant, and self-crossed 9 times by Stougaard.
At Kazusa DNA Res. Inst., genotyping of the RILines is in progress by SSR markers covering the entire genome. A search for the genotype data is available in this database. A search is also available for the phenotypic data evaluated at Miyazaki (N. L. 31° , Long. 131 ° E) and Sapporo (N. L. 43 ° , Long. 141 ° E).